Team newspublished at 11:37 British Summer Time 12 September 2020
Sosaia Feki, Jake Trueman and Cheyse Blair are in the Castleford squad for the rescheduled cup tie, a second game in four days for both clubs.
Andy Last has made eight changes, recalling Hull FC's senior contingent for the game at the TW Stadium.
Jamie Shaul, Carlos Tuimavave, Josh Griffin, Manu Ma'u, Ligi Sao, Jordan Johnstone, Gareth Ellis, and Ratu Naulago add experience to the squad.
Connor Wynne, Josh Bowden, Masi Matongo, Charlie Patterson Lund, Cameron Scott, Ben McNamara, Lewis Bienek and Jude Ferreira all drop out.
Alex Foster and Junior Moors miss out for Castleford, meanwhile, as does the cup-tied Gareth O'Brien.
The match was originally scheduled for 22 August, but was called off after six Hull players tested positive for Covid-19.
Both sides were pushed hard in their Thursday night Super League matches, with Casjust losing out to Warringtonand Hullholding off Wakefield.
Although neither club is out of the play-off race by any means, the Challenge Cup remains an attractive route to success for both, who have been to finals in recent times.
Hull were winners back to back in 2016 and 2017, while Castleford - who could give a much-awaited debut to winter import Feki - reached the 2014 final.
The Airlie Birds have beaten the Tigers in the cup in three of the last five campaigns, including at this same stage last season.