Both second-round ties are all-Championship affairs as Swinton Lions host Oldham and London Broncos face York City Knights.
Swinton come into the game with confidence after beating Newcastle Thunder 28-16 in their first-round tie last weekend. Meanwhile, Oldham saw off League 1 side Barrow Raiders 20-6.
York travel to the capital on the back of a first-round win away at Sheffield Eagles and their opponents London Broncos reached round two with a 24-10 victory against Keighley Cougars.
London BroncosCopyright: London Broncos
London Broncos beat Keighley Cougars in the first round last weekendImage caption: London Broncos beat Keighley Cougars in the first round last weekend
What is the Challenge Cup?
Video content
Video caption: Challenge Cup final highlights: Late Luke Gale drop goal helps Leeds beat SalfordChallenge Cup final highlights: Late Luke Gale drop goal helps Leeds beat Salford
The Challenge Cup is a knockout rugby league cup competition
held annually since 1896, with the exception of 1915–1919 and 1939–1940. It involves
amateur, semi-professional and professional clubs.
Instead, the 13 English clubs from the Championship -
including Bradford, Featherstone and Widnes - were joined by West Wales
and two other clubs from League One.
The 12 Super League clubs will join the competition at the
third-round stage in April, which will be followed by quarter-finals in May,
the semi-finals on 5 June, and the final at Wembley on Saturday, 17 July.
Wigan Warriors are the most successful club in the history
of the competition, winning the cup a record 19 times.
How can I watch?
All times are GMT and subject to change
BBC iPlayer
The BBC will once again bring you live action from the 2021
Challenge Cup, broadcasting two third-round fixtures, two quarter-finals, the
semi-final and final.
You can catch live coverage of the first-round ties - Swinton Lions v Oldham and Featherstone Rovers v Bradford Bulls - on
the Red Button, BBC iPlayer and on the BBC Sport website and app - and if you
miss any of the action, both games will be available for catch-up on the
iPlayer.
Sunday, 28 March
12:20 -14:15 - Swinton Lions v Oldham - BBC Red
Button, BBC iPlayer & BBC Sport website
14:20 -16:15 - London Broncos v York City Knights - BBC Red Button, BBC iPlayer
& BBC Sport website
Inspired to play rugby league?
Get Inspired
#GetInspired
Video content
Video caption: Rugby League: "the speed of an Olympic sprinter and the impact of a car crash"Rugby League: "the speed of an Olympic sprinter and the impact of a car crash"
Fancy giving rugby league a go yourself?
There are so many different versions of the sport, so there
is something to suit everyone, regardless of your age, gender or ability.
Visit our Activity Guide to
find out how you can get started and our Activity Finder to get into rugby
league near you.
Live Reporting
All times stated are UK
London BroncosCopyright: London Broncos London Broncos beat Keighley Cougars in the first round last weekendImage caption: London Broncos beat Keighley Cougars in the first round last weekend Video caption: Challenge Cup final highlights: Late Luke Gale drop goal helps Leeds beat SalfordChallenge Cup final highlights: Late Luke Gale drop goal helps Leeds beat Salford 
Video caption: Rugby League: "the speed of an Olympic sprinter and the impact of a car crash"Rugby League: "the speed of an Olympic sprinter and the impact of a car crash"
Latest PostWho can I watch today?
Both second-round ties are all-Championship affairs as Swinton Lions host Oldham and London Broncos face York City Knights.
Swinton come into the game with confidence after beating Newcastle Thunder 28-16 in their first-round tie last weekend. Meanwhile, Oldham saw off League 1 side Barrow Raiders 20-6.
York travel to the capital on the back of a first-round win away at Sheffield Eagles and their opponents London Broncos reached round two with a 24-10 victory against Keighley Cougars.
What is the Challenge Cup?
Video content
The Challenge Cup is a knockout rugby league cup competition held annually since 1896, with the exception of 1915–1919 and 1939–1940. It involves amateur, semi-professional and professional clubs.
The ongoing implications of the Coronavirus pandemic have forced changes to the structure of the 2021 Challenge Cup because amateur clubs were not able to enter the tournament for the early rounds.
Instead, the 13 English clubs from the Championship - including Bradford, Featherstone and Widnes - were joined by West Wales and two other clubs from League One.
The 12 Super League clubs will join the competition at the third-round stage in April, which will be followed by quarter-finals in May, the semi-finals on 5 June, and the final at Wembley on Saturday, 17 July.
The current holders of the Challenge Cup are Leeds Rhinos, who edged Salford Red Devils 17-16 thanks to a late Luke Gale drop-goal to win the competition for the fourteenth time.
Wigan Warriors are the most successful club in the history of the competition, winning the cup a record 19 times.
How can I watch?
All times are GMT and subject to change
BBC iPlayer
The BBC will once again bring you live action from the 2021 Challenge Cup, broadcasting two third-round fixtures, two quarter-finals, the semi-final and final.
You can catch live coverage of the first-round ties - Swinton Lions v Oldham and Featherstone Rovers v Bradford Bulls - on the Red Button, BBC iPlayer and on the BBC Sport website and app - and if you miss any of the action, both games will be available for catch-up on the iPlayer.
Sunday, 28 March
12:20 -14:15 - Swinton Lions v Oldham - BBC Red Button, BBC iPlayer & BBC Sport website
14:20 -16:15 - London Broncos v York City Knights - BBC Red Button, BBC iPlayer & BBC Sport website
Inspired to play rugby league?
Get Inspired
#GetInspired
Video content
Fancy giving rugby league a go yourself?
There are so many different versions of the sport, so there is something to suit everyone, regardless of your age, gender or ability.
Visit our Activity Guide to find out how you can get started and our Activity Finder to get into rugby league near you.