Let's get more reaction to today's news, this time from Lang Banks, the director of the conservation organisation WWF Scotland, who has been talking to Drivetime.

Banks says: "Despite this news today, I think one thing remains the same - the Scottish government must urgently take steps to address climate change."

Last week, the Scottish government ditched its emissions targets by 75% by 2030.

Lang Banks has welcomed Humza Yousaf saying this morning that tackling climate change remains a priority.

But he added: "The key thing there is delivery."

In the chamber this afternoon, following the break-up of the Bute House agreement, Scottish Greens co-leader Patrick Harvie said: "Who does the first minister think he has pleased most today?

"Is it Douglas Ross, Fergus Ewing or Alex Salmond? And more to the point, which of them does he think he can rely on for a majority in parliament now?"

You can hear more from Patrick Harvie on Radio Scotland's Drivetime after 17:00 and we're expecting the Scottish Greens to hold a press conference in Holyrood's Garden Lobby at 17:30.

Jen Bell from the Rainbow Greens, a wing of the party which represents LGBTQ+ members, told BBC Scotland’s Drivetime that Humza Yousaf has “trampled on democracy".

The Scottish Greens were due to vote in the coming weeks on whether they should remain in power with the SNP before the Bute House agreement was scrapped.

She said: “Our members were keen to have that discussion on the merits of continued co-operation.

“But instead Humza Yousaf has spat at us, he’s turned his back on us and he’s bent the knee to reactionary forces, the oil and gas lobby and the anti-LGBT forces within his own party.”

When asked if she had wanted the power-sharing agreement to continue, she said: “I was open to being convinced."

She said recent issues around the council tax freeze, the scrapping of climate targets, industrial action in the education sector, and the decision to pause the prescription of puberty blockers had brought the Bute House agreement into question.

“We were seeing concession after concession on the issues that mattered to us,” she added.

Image source, Getty Images

As we've been reporting since lunchtime, the Scottish Conservatives have said they will hold a vote of no confidence in First Minister Humza Yousaf next week.

The SNP is now a minority government and will need to win the support of opposition MSPs to get its programme approved by the Scottish Parliament.

Scottish Conservatives chairman Craig Hoy tells Radio Scotland's Drivetime programme that today's events have been a "total and utter humiliation for Humza Yousaf".

He says: "It's now time that Humza Yousaf was dispatched.

"We're very concerned at Humza Yousaf's record as Scotland's first minister."

There is a lot for Scottish Greens co-leaders Lorna Slater and Patrick Harvie to reflect on after the scrapping of the Bute House agreement.

Unlike many workers, their pay packets may not be their number one concern but it's worth pointing out how the decision will affect them.

MSPs receive an annual salary of £72,196, external. This follows a 6.7% pay rise which took effect on 1 April.

Scottish government ministers are entitled to an additional top-up of £33,989 a year, bringing their total salary to £106,185.

For 16 consecutive years, Scottish ministers have declined to accept their full entitlement so their take home pay remains at 2008/9 levels - around £81,449.

Since Scottish Lorna Slater and Patrick Harvie are no longer ministers, they will lose their ministerial allowances and receive the salary of an MSP.

MSPs can claim money back, external for costs incurred in carrying out their duties.

For the 2022/3 financial year, Patrick Harvie claimed £21,344 and Lorna Slater claimed £3,518.

Their business is unfinished but it’s worth reflecting on what the Greens leave behind them on their final day in government.

Patrick Harvie's final ministerial announcement earlier this week was of an £11m package of funds to decarbonise heating in public buildings including Edinburgh University and the Dumfries Ice Bowl.

If it's not scrapped, the most game-changing contribution will be the Heat in Buildings Bill which will require homeowners to change their heating system at the point of sale.

That could have a serious dent on the 15% of our planet-warming greenhouse gas emissions which account for keeping us cosy in our homes.

There's also the recently passed Wildlife and Muirburn Bill, designed to tackle the illegal persecution of birds of prey. And free bus travel for under-22s.

But legacy can be cruelly selective so the party might also be remembered more for some of their failures, like the controversial Highly Protected Marine Areas which fishing communities said would be "catastrophic" for rural areas.

And let's not forget the deposit return scheme for single-use bottles which ended up being shelved because of a row over its implementation with the UK government.

Also at First Minister's Questions, Scottish Liberal-Democrats leader, Alex Cole-Hamilton, said the two partners of this failed agreement were "at each other's throats".

He said: "Two clowns have left the clown car, but this circus continues."

At First Minister's Questions Labour leader Anas Sarwar accused the first minister of "destroying" his party and wanting to "run away" from a general election.

"The first minister is claiming that this is all a sign of strength. The louder he shouts, the weaker he sounds."

He adds that while the challenges facing the country have never been so great, its leadership has "never been so weak".

The SNP have 63 MSPs and the opposition parties together have 65 MSPs.

If the Greens vote against the first minister, he will lose the vote unless he can get the backing of another opposition MSP or other opposition MSPs abstain.

If the Greens abstain rather than voting against him, Humza Yousaf will be safe.

The Greens have yet to decide what they will do, but it’s clear they are very angry.

The other possibility for Humza Yousaf to survive the vote is Alba MSP Ash Regan.

She is a former Scottish government minister and SNP MSP, who stood against Humza Yousaf in the SNP leadership race last year before moving to Alba.

If Humza Yousaf can persuade Ash Regan to back him, that would leave 64 MSPs for him and 64 MSPs against.

In the case of such a tie, the presiding officer casts a deciding vote, which is always for the status quo, meaning Humza Yousaf remaining as first minister.

This could leave the Alba MSP in a strong position to try to strike a deal in return for her support.

First Minister Humza Yousaf is facing a vote of no confidence following the collapse of the SNP power-sharing agreement with the Scottish Greens.

It could take place as early as next week.

The first task is to get 25 MSPs to support the motion. The Scottish Conservatives have 31 MSPs, so we can expect the vote to go ahead.

If a majority of MSPs say they don’t have confidence in the first minister, he would be expected to resign - although he does not have to.

Whether Humza Yousaf will survive the confidence vote is hanging in the balance, with the result probably resting on his former partners, the Scottish Greens or the one Alba MSP.

The Green Party have now confirmed they will hold a press conference in the Garden Lobby of the Scottish Parliament at 17:30 this afternoon.

That's to set out their position on the no-confidence vote in the first minister.

No indication yet as to what that might be - I'm told they will "make their position clear".

You can see the anger on their faces when they speak about what happened this morning.

They had a call late last night summoning them to see the first minister in Bute House at 0800.

The co-leaders - Patrick Harvie and Lorna Slater - were only in for about 10 minutes.

Seething with rage, Patrick Harvie asked the first minister in the chamber at Holyrood "who he could rely on for a majority in parliament?"

The Greens might vote to show their lack of confidence in the first minister - or they could abstain.

Another option could be they announce an opportunity to consult with their members to see what they think.

That's what got them into this position in the first place.

Today's news has been dominated by the Bute House agreement, but what exactly was in it?

In September 2021, the Scottish government and the Scottish Greens entered into a co-operation agreement, external.

The agreement was the first of its type since devolution in Scotland.

The agreement set out how the Scottish government and the Greens intended to work together over five years – the duration of the session six Parliament.

It was announced on 20 August 2021 and subsequently ratified by the Scottish Green Party, external and the national executive committee of the Scottish National Party. SNP members also backed the agreement in a consultative ballot.

Section B of the agreement committed the Scottish government and the Greens to delivering a shared programme which was detailed in the policy programme document Working Together To Build A Greener, Fairer, Independent Scotland., external

This programme set policy priorities in areas such as the climate emergency, economic recovery, child poverty, the natural environment, energy and the constitution.

With thanks to the Scottish Parliament's Information Centre more popularly known as SPICe, external.

Scottish Greens co-leader Patrick Harvie has told Radio 4's World at One programme there was a "difficult exchange of views"with Humza Yousaf this morning.

"There's been a small and mostly fairly marginalised right wing faction on Humza Yousaf's backbenches who've been starting to throw their weight around," he said.

"I think what's happened is that Humza Yousaf has decided to capitulate to that right wing, socially and economically conservative agenda."

He also accused the government of having "dumped" progressive policies from the Bute House Agreement.

He said the Greens would take a collective decision on whether to support a motion of no confidence in the first minister.

As to whether he still had confidence in Humza Yousaf as first minister, he said: "No... we'll have to make a decision on that in light of the first minister's decision to abandon the agreement he made with our party which provided him with a stable majority."

Image source, PA Media

Scotland's former first minister and the leader of the Alba Party Alex Salmond has said that Humza Yousaf has made Alba's Ash Regan the "most powerful MSP in the Scottish parliament".

Speaking to the BBC after the collapse of the SNP's power-sharing agreement with the Greens, Mr Salmond said the first minister had managed to annoy every opposition party in Holyrood.

Ash Regan became Alba’s sole MSP when she resigned from the SNP in October and joined the party led by Alex Salmond.

Ms Regan is writing to the first minister this afternoon to set out her concerns about the Scottish government’s priorities.

She wants to see if there are areas where her party and the SNP can work together ahead of any confidence vote.

It's been a day of strong words across the political spectrum, but here are the things that have still to become clear:

• When will a no confidence vote will be held? MSPs get two sitting days’ notice of a motion of no confidence, meaning the earliest notice could be given is Tuesday with a vote held on Thursday next week.
• How will the Greens and Alba MSP Ash Regan vote? Although the Greens have said the SNP can no longer count on their backing in parliament, they have not yet committed to voting against Humza Yousaf. They could abstain. Ms Regan has not yet indicated her voting intentions either, though she told the BBC she would consider supporting the SNP in parliament on an issue-by-issue basis.
• What has changed since Humza Yousaf said on Saturday that he valued the power-sharing deal with the Greens? When asked whether he could soon be leading a minority government soon, Mr Yousaf had replied: "I don't think that will be the case".
• Will Patrick Harvie remain a co-leader of the Greens? He said he would stand down if party members voted to end the power-sharing deal, but this vote never took place.
• What does a no confidence vote mean for Humza Yousaf's future? If he loses he could reasonably be expected to stand down - but even if he wins, how damaging will this be for his reputation?

• The power-sharing deal between the SNP and the Scottish Greens has been scrapped - a decision made by Humza Yousaf
• It comes just days after he said he valued the agreement and that he did not think he would be leading a minority government soon
• As a result, Green co-leaders Patrick Harvie and Lorna Slater are out of their ministerial posts - they were seen leaving Bute House this morning before an emergency cabinet meeting
• There has been anger among grassroots Green members over the government's decision to scrap key climate targets and over the pausing of puberty blockers for under-18s who were referred to the country's specialist gender clinic
• The Tories will hold a no confidence vote in Humza Yousaf - and given they have 31 MSPs when 25 are needed to support the motion, we can expect the vote to go ahead

In August 2021, just a few months after the Scottish Parliament election, the Scottish government announced it was entering a power-sharing agreement with the Scottish Greens.

The SNP could have operated as a minority government but with the addition of Green MSPs they now had a majority at Holyrood which would alow them to pass budgets, win confidence votes and make legislation - including for another independence referendum.

BBC Scotland political editor Glenn Campbell said at the time: "It looks a lot like a coalition but crucially the Greens have opt-outs from policies they don't like - retaining the ability to criticise the government of which they would become part."

Almost three years later, those policy differences have led to the break up of the power-sharing deal and the removal of Patrick Harvie and Lorna Slater as Scottish Green ministers.

The Alba party's sole MSP, Ash Regan, is expected to write to First Minister Humza Yousaf offering her terms for backing him in the no confidence vote.

Without her vote - or at least a couple of MSPs abstaining or sitting on their hands - his political survival would be in serious doubt.

If all opposition MSPs join forces against the first minister, he will lose.

The SNP have 63 MSPs. Their opponents have 65.

One switcher to the government could result in a tie in which the presiding officer (equivalent of the Commons speaker) would be expected to back "no change".

Image source, Getty Images

The Scottish Greens have been ever-present at the Scottish parliament since devolution.

Robin Harper became the first ever elected Green parliamentarian in the UK after he became an MSP for the Lothians region in the first elections to Holyrood in 1999.

Four years later they added six new MSPs to that total.

That number has fluctuated over the years, but there have always been Scottish Green MSPs in Holyrood.

And in the 2021 election, the party won a record eight seats and entered into a power-sharing agreement with the SNP - which came to an end today.

Image source, Getty Images

Patrick Harvie has served as a co-leader of the Scottish Greens since 2008, and Lorna Slater joined him in 2021.

The party has historically tended to have more than one leader, unlike other traditional political parties.

In recent years, the party has campaigned for rent controls, trans rights, Scottish independence and climate change policies.

Journalist Brian Taylor adds that Yousaf will likely have problems running the country as a minority government.

He says the first minister does not have the numbers to win a no-confidence vote or to get the next budget through unless he does deals with other parties.

"The atmosphere at the Scottish Parliament is now so bitter and astringent that the prospect of deals with other parties is going to be extremely difficult indeed," he says.

Mr Taylor adds that he believes the Greens will vote against the first minister in the confidence vote and it will be a "tough time" for Humza Yousaf.