Vincenzo Montella’s Turkey side had a few chances of their own - Kerem Akturkoglu could not make clean contact a few yards out, and later forced goalkeeper Diogo Costa into a smart save at his near post.
Orkun Kokcu also tested Costa in the first half, while Yusuf Yazici drilled one straight at him in the second half.
Turkey fans were unhappy with the officials, and their players confronted German referee Felix Zwayer, who they felt had awarded Portugal too many free-kicks.
Turkey still have high hopes of qualifying for the knockout stages, with three points in the bag after winning their first match against Georgia.
"We had our own opportunities and were punished at the first mistake. It was a balanced game until the first goal," said Montella.
"These things happen in football. I think we played equal with one of the best teams in Europe. They were much more clinical and that made the difference."
Meanwhile, Portugal's performance only validated their supporters’ hopes that they can go all the way in Germany.
Saturday's game was also tarnished by several people running onto the pitch, bypassing security guards, to try to get pictures with Ronaldo.
The first was a young boy and the Portugal captain posed, but the forward later grew frustrated as others attempted to follow suit.