She tells the audience that there is an NHS guarantee in her manifesto that NHS funding will rise by 2% or by inflation or by Barnett consequentials - whichever is higher. 

Ms Davidson says mental health will be a priority and GP services will receive more funding under her party.

She says her party will bring back prescription charges for 10% of them, leaving 90% free. 

Ms Davidson says all Ms Sturgeon has to do to bring the country together is to a second referendum is off the table for the next parliament, which would provide stability.

Ms Sturgeon says if there is no change in opinion and Scotland is not ripped out of Europe against its will then it will not be on the table.

However, the SNP leader says if a majority of people in Scotland want a referendum then they should get it. 

Ms Davidson says Ms Sturgeon has responsibilities to all of Scotland not just the SNP.

She says Ms Sturgeon "should not be keeping this wound open".

Ms Davidson says the SNP leader has gone back on her promises of once in a lifetime, once in a generation. 

The Scottish Green Party co-convener says we should respect the result of the referendum, but respecting the result also means respecting that a substantial number did vote for independence.

He says the opportunity to continue to debate this is clear.

Mr Harvie says the great mass of people in Scotland are capable of having this debate in a mature way.

The Scottish Lib Dem leader says many areas like education, mental health, climate change and civil liberties have been neglected for the last five years.

Did they not get the message, says Mr Rennie, saying to Ms Sturgeon she is "anti-democratic".

She says if she cannot persuade people that independence is the right option she will not have earned the right to ask the question again.

However, she says if Scotland is ripped out of Europe against its will or the majority of Scots back independence then no politician has the right to stand in the way.

Ms Sturgeon says it is ultimately down to the will of the Scottish people "what is so frightening about that".

Holyrood election 2016: Where the parties stand on Scottish independence.

Image source, PA

The Scottish Labour leader says it's not even 20 months that have passed since the referendum and she believed Nicola Sturgeon when she said it was a once in a lifetime, once in a generation opportunity.

She says Ms Sturgeon is trying to pull the wool over people's eyes.

Ms Dugdale says Ms Sturgeon says "we should trust the pollsters" and if we trusted them Ed Miliband would be prime minister.

Kezia Dugdale accuses Ruth Davidson of telling a porky about the work on the Clyde saying there is an 18 month delay.

Ruth Davidson says the Clyde must not be reliant only on the MOD for contracts.

Nicola Sturgeon again says there is an 18 month delay, to which Ms Davidson says the SNP has been shameful on this issue.

Ms Sturgeon says the unions and workers are furious that promises have not been kept.

A stramash between the two develops.

Willie Rennie asks what Patrick is going to say to the 150,000 people who will lose their jobs in the North Sea.

Mr Harvie says he does not want to leave those over exposed to the same limbo as Longannet and that is why sustainable jobs are vital. 

Partick Harvie says the argument for an energy transition is key.

He says his party will bring relentless pressure to bear to increase public investment.

The Scottish Green Party co-convener says quality employment, as well as quantity, is crucial.

Willie Rennie says a magnet must be created in this country for businesses to invest, to attract growth and opportunity.

He says education must be built up to be the best again and that requires investment for the future.

In order to do that "we need to put a modest penny on income tax", he says.

The priority must be the early years, he adds.

Ms Dugdale says her party opposes the renewal of Trident - but adds thousands of jobs are reliant on that base.

That is why alternative jobs must be provided for these people via a diversification agency, insists the Scottish Labour leader.

She says her party's opposition of Trident is reliant on providing these jobs. 

Scottish Labour leader Kezia Dugdale says "we have to invest" in the skills and development of our people.

Ms Dugdale says her party will use the tax powers of the parliament to raise more money for education to create the jobs necessary.

The Scottish Tory leader says she has spoken to the defence secretary and has been assured there is no change since last year.

Ms Sturgeon insists there is a delay and it is an absolute betrayal.

The Scottish Conservative leader comes back and says there would be no ships built on the Clyde if there was independence.

Ms Davidson adds that one in seven business will be hit by a doubling of the business rates.

She says her party will make sure there are "never taxes higher here" than the rest of the UK.

The Scottish Conservative leader says her party will not increase business rates.