Orlando mayor: Please be patient while we identify victimspublished at 22:42
Although police have started identifying some of those killed, many people are still waiting anxiously for news of their loved ones.
"We know it is a very difficult time for families waiting for information," Orlando Mayor Buddy Dyer said on Facebook.
"As difficult as it is, we ask for patience, compassion and love. Pulse remains an active crime scene. Law enforcement is working the scene as efficiently and diligently as possible while being respectful to the remains of the deceased.
"We are setting up additional means of communication. We have the hotline, 407 246 4357, where it is important if you have a loved one that you believe is a victim to call."