Kate Williams try gives Wales early lead but Italy reply through Sofia Stefan's try and the boot of Michela Sillari, only for Gwenllian Pyrs to score on stroke of half-time to retake lead for Wales.
Francesca Granzotto twice, Silvia Turani, Vittoria Minuzzi and Aura Muzzo cross as Italy take control after break; Wales' misery compounded by Natalia John's late sin bin for head-on-head contact.
Game moved back a day from Saturday due to the funeral of Pope Francis.
Live Reporting
Matt Lloyd
Wales bolster packpublished at 11:04 British Summer Time 27 April
11:04 BST 27 April
Italy v Wales (11:30 BST)
Image source, Huw Evans Picture Agency
Wales head coach Sean Lynn makes three changes to his pack in response to last weekend's disappointing six-try defeat by Ireland.
Prop Donna Rose will make her first start of the campaign, replacing Jenni Scoble who had a tough time in the scrum at Rodney Parade.
Gwen Crabb will look to further steady the pack from the second row, while Georgia Evans, one of Wales' few bright sparks, reverts to number eight.
Vice-captain Alex Callender drops to the bench as Lynn looks to have impact players in a bid to find that elusive 80-minute performance.
Kayleigh Powell is yet to recover from a hamstring injury so Lleucu George remains at fly-half in an unchanged backline.
Wales: Jasmine Joyce-Butchers; Lisa Neumann, Hannah Jones (capt), Courtney Keight, Carys Cox; Lleucu George, Keira Bevan; Gwenllian Pyrs, Kelsey Jones, Donna Rose, Abbie Fleming, Gwen Crabb, Kate Williams, Bethan Lewis, Georgia Evans.
Replacements: Carys Phillips, Maisie Davies, Jenni Scoble, Natalia John, Alex Callender, Sian Jones, Hannah Bluck, Catherine Richards.
Italy change threepublished at 11:02 British Summer Time 27 April
11:02 BST 27 April
Italy v Wales (11:30 BST)
Image source, Getty Images
Image caption,
Scrum-half Sofia Stefan returns
Italy have made three changes.
Sofia Stefan returns at scrum-half, while Francesca Sgorbini and Sara Tounesi ARE back in the Azzurre pack.
Second row Tounesi partners the outstanding Giordana Duca, who along with Wales' Georgia Evans is one of the tournament's top tacklers.
Francesca Granzotto and Isabella Locatelli are also named in the matchday squad having missed the fixtures against France and Scotland while completing their academic studies.
Italy: Vittoria Ostuni Minuzzi; Aura Muzzo, Michela Sillari, Sara Mannini, Alyssa D'Incá; Veronica Madia, Sofia Stefan; Silvia Turani, Vittoria Vecchini, Sara Tounesi, Giordana Duca, Beatrice Veronese, Francesca Sgorbini, Elisa Giordano (capt).